Dr. Sin Hang Lee, a renowned pathologist from Milford, Connecticut, recently created an accurate technique for detecting Ebola virus nucleic acid that involves a nested RT-PCR method.
This method can be used to detect the virus at any of the outbreak sites in West Africa. The non-infection Ebola virus nucleic acid PCR amplicon is convenient and easy to transport to ambient temperatures, where a regional laboratory can confirm the detection.
“Swifter, more efficient and accurate virus testing confirmation will lead to faster patient treatment and isolation at the site of outbreak,” Lee said. “False negative Ebola tests have been reported sporadically after patients have died and the clinical specimens were re-tested. False positive test results are often overlooked, because, if malaria patients were isolated in the same confinement with Ebola patients, the malaria patients might eventually become truly Ebola-positive.”
Lee has made significant other scientific discoveries. He published papers for developing DNA sequencing tests to detect Lyme disease, and he previously created DNA sequencing tests for HPV genotyping at Connecticut’s Milford Hospital.
He announced his new technique at the Pasteur Institute in Paris during a Targeting Ebola meeting on May 28-29.