Experimental vaccine protects monkeys from chikungunya

GALVESTON, Texas — The mosquito-borne virus chikungunya has already infected millions of people in recent outbreaks in South and Southeast Asia, the islands of the Indian Ocean, Africa and northern Italy.

Although seldom fatal, it causes highly painful arthritis-like symptoms that can linger for months or even years. It’s capable of adapting to spread through a mosquito species common in much of North America. And no vaccine or treatment exists to protect humans from its effects.

Chikungunya, from an East African tribal word describing the contorted postures of its pain-wracked victims, has been the focus of intense scientific interest ever since a 2006 outbreak on the island of La Reunion in the Indian Ocean infected about 266,000 people, killing 260 of them.

Now, researchers at the National Institute of Allergy and Infectious Diseases, the University of Texas Medical Branch at Galveston, Purdue University and Bioqual Inc. have developed an experimental vaccine for chikungunya virus and successfully tested it in monkeys.

Described in a paper appearing in the March issue of Nature Medicine, the vaccine is composed of noninfectious “virus-like particles.”

Although coated with the same proteins that enable chikungunya to pass through cell membranes, the vaccine particles lack the proteins that chikungunya uses to replicate inside cells. They look like chikungunya to the immune systems of rhesus macaques, however, which respond to exposure by generating antibodies that defend the monkeys from infection by the real virus.

“This vaccine did an excellent job of protecting the macaques from chikungunya,” said UTMB professor Stephen Higgs, one of the paper’s authors. He was quoted in a press release from the University of Texas Medical Branch at Galveston.

“That it worked so well in a primate model is good news — these macaques are quite similar to humans in their response to chikungunya, and we badly need to develop an effective human vaccine for this virus.”

The researchers used genetic engineering to create the virus-like particles used in the experimental vaccine.

Serum drawn from rhesus macaques injected with the virus-like particles contained substantial levels of antibodies that inactivated chikungunya virus. Two groups of macaques were then inoculated, either with virus-like particles or with a placebo. When the researchers injected the monkeys with chikungunya 15 weeks later, they found that the vaccine had protected the animals from the virus.

Gary Nabel, director of the National Institute of Allergy and Infectious Diseases’ Vaccine Research Center and corresponding author on the Nature Medicine paper, said the vaccine’s effectiveness against chikungunya had led his group to plan follow-up investigations into whether the same approach would work against other alphaviruses, such as Western and Eastern equine encephalitis viruses (both responsible for periodic outbreaks in the United States), and Africa’s o’nyong-nyong virus.